ABSTRACT
Objective:To detect the serum level of chitinase-3-like protein 1 (YKL-40) in patients with pemphigus vulgaris, and to analyze its correlation with the severity of pemphigus vulgaris.Methods:From January 2017 to May 2018, serum samples were collected from 38 patients with pemphigus vulgaris in Department of Dermatology, the Affiliated Hospital of Southwest Medical University, and those collected from 14 age-, gender- and body mass index-matched healthy volunteers served as controls. Serum levels of YKL-40 and Th1/Th2/Th17-related cytokines were detected by using Luminex ? 200 TM system. Mann-Whitney U test was used to compare serum levels of cytokines between the patient group and control group; binary logistic regression was used to investigate factors independently related to the severity of pemphigus vulgaris; a receiver operating characteristic (ROC) curve was drawn, and the area under the curve, sensitivity and specificity were calculated to evaluate the ability of YKL-40 to predict the severity of pemphigus vulgaris. Results:Compared with the control group, the patient group showed significantly increased serum levels of YKL-40 (expressed as median[ Q1, Q3]: 15.22 [14.19, 15.93] vs. 13.64 [13.21, 14.63]μg/L, z=-3.88, P < 0.05) , interleukin (IL) -6 (2.05 [1.49, 4.21] vs. 1.57[1.38, 1.75]ng/L, z=-2.44, P < 0.05) , IL-7 (7.45[5.63, 11.63] vs. 3.77[2.21, 5.97]ng/L, z=-3.26, P < 0.05], IL-8 (6.59[3.60, 14.73] vs. 4.36[2.96, 6.53]ng/L, z=-1.96, P < 0.05) , IL-2R-α (509.08 [386.36, 757.67] vs. 336.44[309.86, 458.71]ng/L, z=-2.35, P < 0.05) , and C5a (100.35 [78.31, 140.84] vs. 72.08 [37.23, 82.08] ng/L, z = -3.04, P < 0.05) . The concentration of serum YKL-40 gradually decreased along with the reduction of lesion areas ( r = 0.63, P < 0.001) , and YKL-40 was independently correlated with the severity of pemphigus vulgaris ( P = 0.025, OR = 46.54, 95% CI: 1.61, 1 347.19) . The area under the curve of YKL-40 was 0.783 (95% CI: 0.613, 0.953) for distinguishing between patients with severe to extremely severe pemphigus vulgaris and those with mild to moderate pemphigus vulgaris. Conclusion:The serum level of YKL-40 is strongly correlated with the severity of pemphigus vulgaris, and has a potential value in predicting the severity of this disease.
ABSTRACT
Objective To observe the clinical efficacy of dye laser combined with Xin'an repair solution in the treating superficial infantile hemangioma(IH). Methods 100 patients with superficial IH were randomly divided into observation group and control group.The observation group received dye laser combined with Xin'an re-pair solution,while the control group was treated only with dye laser.The clinical efficacy and the rate of complica-tions after treatment were compared between the two groups,and the different efficacy between the head neck group and the non head neck group in the observation group was further analyzed. Results The total effective rate of the observation group(90%)was significantly higher than the control group(70%),and the difference was sta-tistically significant(P<0.05).The rate of complications in the observation group was significantly lower than that in the control group(6%vs.32%,P<0.05).In the observation group,the total effective rate of head neck group was better than that the non head neck group(P<0.05). Conclusion Dye laser combined with Xin'an repair so-lution is effective in the treatment of superficial IH,and it can significantly reduce complications.It is evidently ef-fective for head and neck superficial IH.
ABSTRACT
BACKGROUND: The expression of matrix metalloproteinases (MMPs) is enhanced in fibroblasts of aging skin; tripterine and ginsenoside Rd possess immunosuppression and anti-aging effects. Arotinoid ethyl ester can used to treat sun-induced aging skin, which accompanied by many side effects, OBJECTIVE: To discuss the regulation role of ginsenoside Rd, celastrol, arotinoid ethyl ester on MMP-1 and MMP-3 of the cultured fibroblasts of the human dermis in vitro. METHODS: The human dermis circumcision by surgical excision on newborn was obtained from Department of Surgery, Luzhou Medical College, and the informed consent obtained from patients. Culture of fibroblasts were as follow: ①No treatment in the normal control group. ②Radiated with 80 kJ/m~2 ultraviolet and treated by 100mg/L8-methoxypsoralen in the positive control group. ③Treated by ultraviolet radiation+8-methoxypsoralen+arotinoid ethyl ester in the arotinoid ethyl ester group. ④Treated by ultraviolet radiation +8-methoxypsoralen+triptolide in the triptolide group. ⑤Treated byultraviolet radiation+8-methoxypsoralen+ginsenoside Rd in the ginsenoside Rdgroup. The triptolide group was divided into 3 groups with doses of 10, 20, and 40 mg/L. The ginsenoside Rd group was divided into 3 groups with doses of 20, 50 and 100 mg/L. The immunohistochemistry staining of MMP-1 and MMP-3 of the fibroblasts in each group was observed. RESULTS and CONCLUSION: The expressions of MMP-1 and MMP-3 were significantly increased in the positive control group, which had a significant difference to the normal control group (P < 0.05). Compared to the positive control group, the expression of MMP-1 and MMP-3 significantly decreased in the treatment groups (P < 0.05); however, the differences among each treatment groups had no significance (P > 0.05). The results demonstrated that:①Ginsenoside Rd and tripterine exhibit comparably effect on prevent and cure sun-induced aging skin.②The wide ranges of effective concentration lead to little side effect to the body. ③The action mechanisms of ginsenoside Rd, celastrol and arotinoid ethyl ester in preventing and curing sun-induced aging skin is adjusting the expressions of MMP-1 and MMP-3.
ABSTRACT
BACKGROUND:The expression of matrix metalloproteinases (MMPs) is enhanced in fibroblasts of aging skin; tripterine and ginsenoside Rd possess immunosuppression and anti-aging effects. Arotinoid ethyl ester can used to treat sun-induced aging skin,which accompanied by many side effects. OBJECTIVE:To discuss the regulation role of ginsenoside Rd,celastrol,arotinoid ethyl ester on MMP-1 and MMP-3 of the cultured fibroblasts of the human dermis in vitro. METHODS:The human dermis circumcision by surgical excision on newborn was obtained from Department of Surgery,Luzhou Medical College,and the informed consent obtained from patients. Culture of fibroblasts were as follow:①No treatment in the normal control group. ②Radiated with 80 kJ/m2 ultraviolet and treated by 100 mg/L 8-methoxypsoralen in the positive control group. ③Treated by ultraviolet radiation+8-methoxypsoralen+arotinoid ethyl ester in the arotinoid ethyl ester group. ④Treated by ultraviolet radiation +8-methoxypsoralen+triptolide in the triptolide group. ⑤Treated by ultraviolet radiation+8-methoxypsoralen+ginsenoside Rd in the ginsenoside Rd group. The triptolide group was divided into 3 groups with doses of 10,20,and 40 mg/L. The ginsenoside Rd group was divided into 3 groups with doses of 20,50 and 100 mg/L. The immunohistochemistry staining of MMP-1 and MMP-3 of the fibroblasts in each group was observed. RESULTS and CONCLUSION:The expressions of MMP-1and MMP-3 were significantly increased in the positive control group,which had a significant difference to the normal control group (P 0.05). The results demonstrated that:①Ginsenoside Rd and tripterine exhibit comparably effect on prevent and cure sun-induced aging skin. ②The wide ranges of effective concentration lead to little side effect to the body. ③ The action mechanisms of ginsenoside Rd,celastrol and arotinoid ethyl ester in preventing and curing sun-induced aging skin is adjusting the expressions of MMP-1 and MMP-3.